Microarray normalization online dating
In 2-channel microarray experiments, you have 2 samples (e.g., collected under condition A and under condition B) of nucleic acid, with one sample labeled with a red (R) fluorescent marker and the other with green (G).
The samples are mixed and applied to an array of spots, with each individual spot binding a specific nucleic-acid sequence.
Thankyou @Edm for you answer,however it's not quite clear for me. the measured signal is something like this: mean(biological variance) mean(technical variance) constant biases variabele biases.
I still don't understand which of these normalization corrects for?
Dates First available in Project Euclid: 21 January 2005Permanent link to this documenthttps://projecteuclid.org/euclid.bj/1106314844Digital Object Identifierdoi:10.3150/bj/1106314844Mathematical Reviews number (Math Sci Net) MR2108037Zentralblatt MATH identifier1064.62111Keywordsgene expression intensity dependence MA-plot microarray normalization Ting Lee, Mei-Ling; Whitmore, George A.
Intensity-dependent normalization in microarray analysis: a note of concern.
For commercial re-use, please contact [email protected] Gene Expression Profile Analysis Suite (GEPAS) has been running for more than four years.The web-based pipeline for microarray gene expression data, GEPAS, is available at The online version of this article has been published under an open access model.Now let's examine different labeling strategies for performing 6 replicates of this experiment.First, say that all samples from condition A are labeled G and all from condition B are labeled R.
The Gene Expression Profile Analysis Suite (GEPAS) has been running for more than four years.